The lectins griffithsin, cyanovirin-N and scytovirin inhibit HIV-1 binding to the DC-SIGN receptor and transfer to CD4(+) cells.
Virology
; 423(2): 175-86, 2012 Feb 20.
Article
en En
| MEDLINE
| ID: mdl-22209231
It is generally believed that during the sexual transmission of HIV-1, the glycan-specific DC-SIGN receptor binds the virus and mediates its transfer to CD4(+) cells. The lectins griffithsin (GRFT), cyanovirin-N (CV-N) and scytovirin (SVN) inhibit HIV-1 infection by binding to mannose-rich glycans on gp120. We measured the ability of these lectins to inhibit both the HIV-1 binding to DC-SIGN and the DC-SIGN-mediated HIV-1 infection of CD4(+) cells. While GRFT, CV-N and SVN were moderately inhibitory to DC-SIGN binding, they potently inhibited DC-SIGN-transfer of HIV-1. The introduction of the 234 glycosylation site abolished HIV-1 sensitivity to lectin inhibition of binding to DC-SIGN and virus transfer to susceptible cells. However, the addition of the 295 glycosylation site increased the inhibition of transfer. Our data suggest that GRFT, CV-N and SVN can block two important stages of the sexual transmission of HIV-1, DC-SIGN binding and transfer, supporting their further development as microbicides.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas Bacterianas
/
Linfocitos T CD4-Positivos
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Proteínas Portadoras
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Moléculas de Adhesión Celular
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Regulación hacia Abajo
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Infecciones por VIH
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VIH-1
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Receptores de Superficie Celular
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Proteínas Algáceas
/
Lectinas Tipo C
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2012
Tipo del documento:
Article
País de afiliación:
Sudáfrica
Pais de publicación:
Estados Unidos