Obscurin depletion impairs organization of skeletal muscle in developing zebrafish embryos.
J Biomed Biotechnol
; 2011: 479135, 2011.
Article
en En
| MEDLINE
| ID: mdl-22190853
During development, skeletal myoblasts differentiate into myocytes and skeletal myotubes with mature contractile structures that are precisely oriented with respect to surrounding cells and tissues. Establishment of this highly ordered structure requires reciprocal interactions between the differentiating myocytes and the surrounding extracellular matrix to form correctly positioned and well-organized attachments from the skeletal muscle to the bony skeleton. Using the developing zebrafish embryo as a model, we examined the relationship between new myofibril assembly and the organization of the membrane domains involved in cell-extracellular matrix interactions. We determined that depletion of obscurin, a giant muscle protein, resulted in irregular cell morphology and disturbed extracellular matrix organization during skeletal muscle development. The resulting impairment of myocyte organization was associated with disturbance of the internal architecture of the myocyte suggesting that obscurin participates in organizing the internal structure of the myocyte and translating those structural cues to surrounding cells and tissues.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Diferenciación Celular
/
Fibras Musculares Esqueléticas
/
Factores de Intercambio de Guanina Nucleótido
/
Proteínas de Pez Cebra
/
Desarrollo de Músculos
/
Mioblastos Esqueléticos
/
Embrión no Mamífero
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
J Biomed Biotechnol
Asunto de la revista:
BIOTECNOLOGIA
/
MEDICINA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos