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The localisation of HSP70 and oxidative stress indices in heads of Spodoptera exigua larvae in a cadmium-exposed population.
Kafel, Alina; Nowak, Aleksandra; Bembenek, Jadwiga; Szczygiel, Joanna; Nakonieczny, Miroslaw; Swiergosz-Kowalewska, Renata.
Afiliación
  • Kafel A; University of Silesia, Department of Animal Physiology and Ecotoxicology, Bankowa 9, PL 40-007, Katowice, Poland. alina.kafel@us.edu.pl
Ecotoxicol Environ Saf ; 78: 22-7, 2012 Apr.
Article en En | MEDLINE | ID: mdl-22133653
The effects of cadmium toxicity may vary between animals with different history of metal exposure. The aim of our study was to examine HSP70, protein carbonyl levels, catalase activity and total antioxidant capacity in the heads of Spodoptera exigua (Hübner) larvae originated from undergoing 1- and 44-generational cadmium treatment and in control (those that were not exposed to cadmium). We also measured the cadmium concentration and DNA damage level in the larvae. We observed higher level of heat shock proteins (HSPs) in the heads of larvae derived from multi-generational metal treatment than in the heads of those from one-generational treatment (derived from the control rearing). Analysis of HSP localisation in the larval brain suggests that these changes could be important for protecting the neural function of larval mushroom bodies for animals selected during multigenerational metal exposure. Animals from one-generational treatment had, in turn, higher total antioxidant capacity than animals from multigenerational treatment. Anyway, animals from one- and 44-generational metal treatments did not differ in metal accumulation in the heads and the whole larval bodies, catalase activity or DNA damage level. All these measurements were higher than for control larvae and cadmium accumulation in the heads was much lower than in the whole bodies.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes del Suelo / Cadmio / Spodoptera / Proteínas HSP70 de Choque Térmico Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2012 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Contaminantes del Suelo / Cadmio / Spodoptera / Proteínas HSP70 de Choque Térmico Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Ecotoxicol Environ Saf Año: 2012 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos