Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous.
Mol Vis
; 17: 2856-63, 2011.
Article
en En
| MEDLINE
| ID: mdl-22128233
PURPOSE: Recent studies have illuminated the vitreous proteome as a potentially important diagnostic tool that will predict disease progression and response to treatment, in eyes with retinal disease. Studies to date have demonstrated correlations of protein levels between vitreous and aqueous humor. Because these results are un-expected and analysis was only done on a few endpoints, the present study further analyzes the relationship between aqueous and vitreous by probing a wide array of proteins in patients with posterior segment diseases. METHODS: Anterior chamber aqueous fluid was obtained using a limbal approach with a 30 gauge needle. Immediately following, the vitreous sample was obtained via a pars plana approach. A 25 gauge needle with a 1 ml syringe was directed into the mid-vitreous cavity and vitreous fluid was gently aspirated. Aqueous and vitreous samples were then analyzed using the quantitative native protein analysis method called reverse phase protein microarray technology (RPPM). RESULTS: The entire sample population (n=11) was probed against 34 proteins, revealing 8 proteins that significantly correlate, 3 proteins that trend to correlation but fell short of significance and 23 proteins that have no correlation between the vitreous and aqueous humor. CONCLUSIONS: Proteins in the aqueous cannot be assumed to correlate with their counterparts in the vitreous.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Humor Acuoso
/
Enfermedades de la Retina
/
Vitrectomía
/
Cuerpo Vítreo
/
Expresión Génica
/
Citocinas
/
Péptidos y Proteínas de Señalización Intercelular
Tipo de estudio:
Prognostic_studies
Límite:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Mol Vis
Asunto de la revista:
BIOLOGIA MOLECULAR
/
OFTALMOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos