Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-beta-mediated epithelial-mesenchymal transition.
Int J Radiat Oncol Biol Phys
; 81(5): 1530-7, 2011 Dec 01.
Article
en En
| MEDLINE
| ID: mdl-22115555
PURPOSE: To examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-ß)-mediated epithelial-mesenchymal transition (EMT). METHODS AND MATERIALS: Six cancer cell lines originating from different human organs were irradiated by 60Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-ß in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-ß signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. RESULTS: After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-ß were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-ß signaling. CONCLUSIONS: These results suggest that EMT mediated by TGF-ß plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Movimiento Celular
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Factor de Crecimiento Transformador beta1
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Transición Epitelial-Mesenquimal
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Invasividad Neoplásica
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Metástasis de la Neoplasia
Límite:
Humans
Idioma:
En
Revista:
Int J Radiat Oncol Biol Phys
Año:
2011
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Estados Unidos