Sensitive and specific single-molecule sequencing of 5-hydroxymethylcytosine.

Song, Chun-Xiao; Clark, Tyson A; Lu, Xing-Yu; Kislyuk, Andrey; Dai, Qing; Turner, Stephen W; He, Chuan; Korlach, Jonas

Song, Chun-Xiao; Clark, Tyson A; Lu, Xing-Yu; Kislyuk, Andrey; Dai, Qing; Turner, Stephen W; He, Chuan; Korlach, Jonas.

Afiliación

Song CX; Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, Chicago, Illinois, USA.

Nat Methods ; 9(1): 75-7, 2011 Nov 20.

Article en En | MEDLINE | ID: mdl-22101853

RESUMEN

We describe strand-specific, base-resolution detection of 5-hydroxymethylcytosine (5-hmC) in genomic DNA with single-molecule sensitivity, combining a bioorthogonal, selective chemical labeling method of 5-hmC with single-molecule, real-time (SMRT) DNA sequencing. The chemical labeling not only allows affinity enrichment of 5-hmC-containing DNA fragments but also enhances the kinetic signal of 5-hmC during SMRT sequencing. We applied the approach to sequence 5-hmC in a genomic DNA sample with high confidence.

Asunto(s)

Citosina/análogos & derivados; ADN/química; Análisis de Secuencia de ADN/métodos; 5-Metilcitosina/análogos & derivados; Secuencia de Bases; Citosina/análisis; Sensibilidad y Especificidad

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Análisis de Secuencia de ADN / Citosina Tipo de estudio: Diagnostic_studies Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2011 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

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