In-cell click labelling of small molecules to determine subcellular localisation.
J Chem Biol
; 4(2): 49-53, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-22096474
UNLABELLED: Small molecule fluorometric boron dipyrromethene probes were developed to bind hepatitis C virus-encoded NS5A protein and aid subcellular distribution studies. These molecules did not co-locate with NS5A, therefore alternative 'silent' azide reporters were used to obtain a more relevant picture of their distribution. Following pre-incubation with replicon cells, click chemistry was used to append a fluorophore to the azide that confirmed the co-localisation of the small molecule with the NS5A protein, thus providing greater insight into the antiviral mode of action of this chemotype. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s12154-010-0047-1) contains supplementary material, which is available to authorized users.
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1
Colección:
01-internacional
Base de datos:
MEDLINE
Idioma:
En
Revista:
J Chem Biol
Año:
2011
Tipo del documento:
Article
Pais de publicación:
Alemania