Consequences of butylated hydroxytoluene in the freezing extender on post-thaw characteristics of stallion spermatozoa in vitro.
Andrologia
; 44 Suppl 1: 688-95, 2012 May.
Article
en En
| MEDLINE
| ID: mdl-22082046
Ejaculates from six pure Spanish stallions were split, and one subsample frozen in a commercial extender supplemented with the lipid soluble antioxidant butylated hydroxytoluene (BHT), while the other subsample served as control. After at least 4 weeks of storage, samples were thawed and post-thaw sperm quality analysed: sperm motility and kinematics using a CASA system, membrane and acrosome integrity and mitochondrial membrane potential using flow cytometry. The outcome of cryopreservation varied significantly among stallions. However, the supplementation with 1 mm BHT had no significant effect on any of the sperm parameters evaluated post-thaw.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Preservación de Semen
/
Espermatozoides
/
Hidroxitolueno Butilado
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Criopreservación
/
Congelación
Límite:
Animals
Idioma:
En
Revista:
Andrologia
Año:
2012
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Alemania