Isolation of Candida tropicalis auxotrophic mutants.

Gleeson, M A; Haas, L O; Cregg, J M

Gleeson, M A; Haas, L O; Cregg, J M.

Afiliación

Gleeson MA; Salk Institute Biotechnology/Industrial Associates, Inc., La Jolla, California 92037.

Appl Environ Microbiol ; 56(8): 2562-4, 1990 Aug.

Article en En | MEDLINE | ID: mdl-2206102

RESUMEN

An enrichment scheme using nystatin was designed for the isolation of auxotrophic mutants from the diploid-alkane-utilizing yeast Candida tropicalis. A collection of 194 auxotrophs representing 7 phenotypes was isolated. One class of mutants was identified as having a defect in histidinol dehydrogenase activity and a second class of mutants was identified as having a defect in orotidine monophosphate decarboxylase activity. These strains are good candidates to be carrying mutations corresponding to the HIS4 and URA3 genes of Saccharomyces cerevisiae.

Asunto(s)

Candida/genética; Oxidorreductasas de Alcohol/genética; Candida/enzimología; Candida/aislamiento & purificación; Marcadores Genéticos; Mutación; Micología/métodos; Nistatina; Orotidina-5'-Fosfato Descarboxilasa/genética; Saccharomyces cerevisiae/genética; Especificidad de la Especie

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Candida Idioma: En Revista: Appl Environ Microbiol Año: 1990 Tipo del documento: Article Pais de publicación: Estados Unidos

Texto completo

Añadir a Mi BVS

Imprimir

XML

PubMed Links

Buscar en Google