Palladium(II) and platinum(II) bind strongly to an engineered blue copper protein.
Inorg Chem
; 50(22): 11294-6, 2011 Nov 21.
Article
en En
| MEDLINE
| ID: mdl-22026434
Studies of palladium(II) and platinum(II) binding to well-characterized proteins contribute to understanding the influence of these metals in the environment and body. The well-characterized apoprotein of azurin has a soft-metal binding site that may be exposed to solvent by mutation of a coordinating His-117 residue to glycine (H117G). Palladium(II) and platinum(II) form strong 1:1 adducts with the apo form of H117G azurin. A combination of UV-vis, circular dichroism, and inductively coupled plasma mass spectrometry techniques suggests that the metal binds specifically at His-46 and Cys-112 of the protein.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Paladio
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Platino (Metal)
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Pseudomonas aeruginosa
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Azurina
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Proteínas Bacterianas
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Cobre
Idioma:
En
Revista:
Inorg Chem
Año:
2011
Tipo del documento:
Article
País de afiliación:
Estados Unidos
Pais de publicación:
Estados Unidos