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Isolated in vitro brainstem-spinal cord preparations remain important tools in respiratory neurobiology.
Johnson, Stephen M; Turner, Sara M; Huxtable, Adrianne G; Ben-Mabrouk, Faiza.
Afiliación
  • Johnson SM; Department of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA. johnsons@svm.vetmed.wisc.edu
Respir Physiol Neurobiol ; 180(1): 1-7, 2012 Jan 15.
Article en En | MEDLINE | ID: mdl-22015642
Isolated in vitro brainstem-spinal cord preparations are used extensively in respiratory neurobiology because the respiratory network in the pons and medulla is intact, monosynaptic descending inputs to spinal motoneurons can be activated, brainstem and spinal cord tissue can be bathed with different solutions, and the responses of cervical, thoracic, and lumbar spinal motoneurons to experimental perturbations can be compared. The caveats and limitations of in vitro brainstem-spinal cord preparations are well-documented. However, isolated brainstem-spinal cords are still valuable experimental preparations that can be used to study neuronal connectivity within the brainstem, development of motor networks with lethal genetic mutations, deleterious effects of pathological drugs and conditions, respiratory spinal motor plasticity, and interactions with other motor behaviors. Our goal is to show how isolated brainstem-spinal cord preparations still have a lot to offer scientifically and experimentally to address questions within and outside the field of respiratory neurobiology.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Órganos / Fenómenos Fisiológicos Respiratorios / Médula Espinal / Tronco Encefálico / Neurobiología Límite: Animals Idioma: En Revista: Respir Physiol Neurobiol Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo de Órganos / Fenómenos Fisiológicos Respiratorios / Médula Espinal / Tronco Encefálico / Neurobiología Límite: Animals Idioma: En Revista: Respir Physiol Neurobiol Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos