Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms.
Physiol Res
; 60(6): 899-904, 2011.
Article
en En
| MEDLINE
| ID: mdl-21995898
We developed a new method for the quantitative determination of myosin heavy chain (MyHC) isoforms taking advantage of immunochemical differences and based on the ELISA principle. In the present paper we compare analysis of MyHC isoforms using the SDS-PAGE and the ELISA methods in the same samples of adult female inbred Lewis strain euthyroid, hyperthyroid and hypothyroid rats. In all thyroid states, the same composition and corresponding changes of MyHC isoforms were determined using both methodological approaches in the slow soleus and the fast extensor digitorum longus muscles. Our results showed that ELISA can be used for a "semi-quantitative" or "comparative" measurement of MyHC isoforms in multiple muscle samples, but that it is neither more exact nor faster compared to SDS-PAGE.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Cadenas Pesadas de Miosina
Límite:
Animals
Idioma:
En
Revista:
Physiol Res
Asunto de la revista:
FISIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
República Checa
Pais de publicación:
República Checa