Prevalence of extended-spectrum ß-lactamase-producing Escherichia coli and Klebsiella pneumoniae in food-producing animals.

Hiroi, Midori; Yamazaki, Fumie; Harada, Tetsuya; Takahashi, Naomi; Iida, Natsuko; Noda, Yoshihiro; Yagi, Miya; Nishio, Tomohiro; Kanda, Takashi; Kawamori, Fumihiko; Sugiyama, Kanji; Masuda, Takashi; Hara-Kudo, Yukiko; Ohashi, Norio

Hiroi, Midori; Yamazaki, Fumie; Harada, Tetsuya; Takahashi, Naomi; Iida, Natsuko; Noda, Yoshihiro; Yagi, Miya; Nishio, Tomohiro; Kanda, Takashi; Kawamori, Fumihiko; Sugiyama, Kanji; Masuda, Takashi; Hara-Kudo, Yukiko; Ohashi, Norio.

Afiliación

Hiroi M; Department of Microbiology, Shizuoka Institute of Environment and Hygiene, 4272 Kitaando, Aoi, Shizuoka 4208637, Japan. midori1_hiroi@pref.shizuoka.lg.jp

J Vet Med Sci ; 74(2): 189-95, 2012 Feb.

Article en En | MEDLINE | ID: mdl-21979457

RESUMEN

To evaluate the diversity of extended-spectrum ß-lactamases (ESBL) genes among food-producing animals, 48 isolates of ESBL-producing Escherichia coli isolates were obtained from rectal samples of broilers, layers, beef cattle and pigs, at the slaughterhouse level. ESBL-carrying E. coli were isolated from 60.0% of individual broiler rectal samples, 5.9% of layers, 12.5% of beef cattle and 3% of pigs. One ESBL-producing Klebsiella pneumoniae was isolated from a broiler. The ESBL-positive E. coli isolates from broilers harbored various ESBL genes: bla (SHV-12), bla(CTX-M-2), bla(CTX-M-14), bla(CTX-M-15) and bla(CTX-M-44). The plasmid DNAs were analyzed by restriction patterns. Homogeneous band patterns were yielded in those of K. pneumoniae and E. coli isolates harboring the bla(CTX-M-2) gene from different farms. No genetic relation between the 2 CTX-M-14 ESBL-producing strains was found by pulsed-field gel electrophoresis, although 2 plasmids in these strains, obtained from different broiler farms, were similar to each other. This study provides evidence that the proliferation of CTX-M-producing E. coli is due to the growth of indigenous CTX-M-producing strains and the possible emergence of strains that acquired CTX-M genes by horizontal transfer in different broiler farms. CTX-M-producing coliforms in broilers should be controlled due to the critical importance of cephalosporins and the zoonotic potential of ESBL-producing bacteria.

Asunto(s)

Escherichia coli/enzimología; Microbiología de Alimentos; Klebsiella pneumoniae/enzimología; beta-Lactamasas/aislamiento & purificación; Animales; Bovinos; Pollos; ADN Bacteriano/química; ADN Bacteriano/genética; Electroforesis en Gel de Campo Pulsado/veterinaria; Escherichia coli/genética; Heces/microbiología; Klebsiella pneumoniae/genética; Pruebas de Sensibilidad Microbiana/veterinaria; Plásmidos/química; Plásmidos/genética; Reacción en Cadena de la Polimerasa/veterinaria; Porcinos; beta-Lactamasas/biosíntesis; beta-Lactamasas/genética

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Beta-Lactamasas / Escherichia coli / Microbiología de Alimentos / Klebsiella pneumoniae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: J Vet Med Sci Asunto de la revista: MEDICINA VETERINARIA Año: 2012 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón

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