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Monitoring of artemisinin, dihydroartemisinin, and artemether in environmental matrices using high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS).
Jessing, Karina Knudsmark; Juhler, Renè K; Strobel, Bjarne Westergaard.
Afiliación
  • Jessing KK; Department of Basic Sciences and Environment, University of Copenhagen, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark. jessing@life.ku.dk
J Agric Food Chem ; 59(21): 11735-43, 2011 Nov 09.
Article en En | MEDLINE | ID: mdl-21961706
The area cultivated with Artemisia annua for the extraction of the antimalarial compound artemisinin is increasing, but the environmental impact of this cultivation has not yet been studied. A sensitive and robust method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of artemisinin in soil. Dihydroartemisinin and artemether were included in the method, and performance on analytical columns of both traditional C(18) phenyl-hexyl and porous shell particles-based Kinetex types was characterized. The versatility of the method was demonstrated on surface water and groundwater samples and plant extracts. The limit of detection was 55, 30 (25 ng/g soil), and 4 ng/mL for dihydroartemisinin, artemisinin, and artemether, respectively. Method performance was demonstrated using naturally contaminated soil samples from A. annua fields in Kenya. The highest observed concentrations were above EC(10) for lettuce growth. Monitoring of artemisinin in soil with A. annua crop production seems necessary to further understand the impact in the environment.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Cromatografía Líquida de Alta Presión / Artemisia annua / Artemisininas / Espectrometría de Masas en Tándem Tipo de estudio: Evaluation_studies País/Región como asunto: Africa Idioma: En Revista: J Agric Food Chem Año: 2011 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Extractos Vegetales / Cromatografía Líquida de Alta Presión / Artemisia annua / Artemisininas / Espectrometría de Masas en Tándem Tipo de estudio: Evaluation_studies País/Región como asunto: Africa Idioma: En Revista: J Agric Food Chem Año: 2011 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Estados Unidos