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Designing phenotyping studies for genetically engineered mice.
Zeiss, C J; Ward, J M; Allore, H G.
Afiliación
  • Zeiss CJ; Section of Comparative Medicine, Yale University School of Medicine, TAC N230, New Haven, CT 06520, USA. caroline.zeiss@yale.edu
Vet Pathol ; 49(1): 24-31, 2012 Jan.
Article en En | MEDLINE | ID: mdl-21930803
A phenotyping study records physiologic or morphologic changes in an experimental animal resulting from an intervention. In mice, this intervention is most frequently genetic, but it may be any type of experimental manipulation. Accurate representation of the human condition under study is essential if the model is to yield useful conclusions. In this review, general approaches to the design of phenotyping studies are considered. These approaches take into account major sources of reduced model validity, such as unexpected phenotypic variation in mice, evolutionary divergence between mice and humans, unanticipated sources of variation, and common design errors. As poor design is the most common reason why studies fail to yield enduring results, emphasis is placed on reduction of bias, sampling, controlled study design, and appropriate statistical analysis.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Proyectos de Investigación / Ratones Transgénicos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Vet Pathol Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fenotipo / Proyectos de Investigación / Ratones Transgénicos Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Vet Pathol Año: 2012 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos