Insights into subtle conformational differences in the substrate-binding loop of fungal 17ß-hydroxysteroid dehydrogenase: a combined structural and kinetic approach.
Biochem J
; 441(1): 151-60, 2012 Jan 01.
Article
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| MEDLINE
| ID: mdl-21929506
The 17ß-HSD (17ß-hydroxysteroid dehydrogenase) from the filamentous fungus Cochliobolus lunatus (17ß-HSDcl) is a NADP(H)-dependent enzyme that preferentially catalyses the interconversion of inactive 17-oxo-steroids and their active 17ß-hydroxy counterparts. 17ß-HSDcl belongs to the SDR (short-chain dehydrogenase/reductase) superfamily. It is currently the only fungal 17ß-HSD member that has been described and represents one of the model enzymes of the cP1 classical subfamily of NADPH-dependent SDR enzymes. A thorough crystallographic analysis has been performed to better understand the structural aspects of this subfamily and provide insights into the evolution of the HSD enzymes. The crystal structures of the 17ß-HSDcl apo, holo and coumestrol-inhibited ternary complex, and the active-site Y167F mutant reveal subtle conformational differences in the substrate-binding loop that probably modulate the catalytic activity of 17ß-HSDcl. Coumestrol, a plant-derived non-steroidal compound with oestrogenic activity, inhibits 17ß-HSDcl [IC50 2.8 µM; at 100 µM substrate (4-oestrene-3,17-dione)] by occupying the putative steroid-binding site. In addition to an extensive hydrogen-bonding network, coumestrol binding is stabilized further by π-π stacking interactions with Tyr212. A stopped-flow kinetic experiment clearly showed the coenzyme dissociation as the slowest step of the reaction and, in addition to the low steroid solubility, it prevents the accumulation of enzyme-coenzyme-steroid ternary complexes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ascomicetos
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Regulación Enzimológica de la Expresión Génica
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Regulación Fúngica de la Expresión Génica
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Hidroxiesteroide Deshidrogenasas
Idioma:
En
Revista:
Biochem J
Año:
2012
Tipo del documento:
Article
País de afiliación:
Italia
Pais de publicación:
Reino Unido