Chemical characterization of lipopolysaccharides from Proteus strains used in Weil-Felix test.
Microbiol Immunol
; 34(2): 121-33, 1990.
Article
en En
| MEDLINE
| ID: mdl-2189059
The lipopolysaccharides (LPS) extracted from Proteus strains OX2, OX19, and OXK used as antigens in the Weil-Felix test, were characterized by chemical analysis and SDS-polyacrylamide gel electrophoresis (SDS-PAGE). To separate the O-polysaccharide, core-oligosaccharide, and lipid A moieties, each LPS was treated with 2% acetic acid, centrifuged, and applied to Sephadex G-50 column. The core-oligosaccharides contained L-glycero-D-mannoheptose, D-glycero-D-mannoheptose, glucose (Glc), galactose, 3-deoxy-D-mannooctulosonic acid, uronic acid, phosphate, glucosamine (GlcN), and galactosamine (GalN). The lipid A preparations contained GlcN, GlcN-phosphate, and three fatty acids (myristic, plamitic, and beta-hydroxymyristic acids). However, the O-polysaccharides of OX2- and OXK-LPS had different chemical compositions which consisted of Glc, GlcN, and quinovosamine, and Glc, uronic acid, and GalN, respectively, while OX19-LPS seemed to lack O-polysaccharide.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteus
/
Lipopolisacáridos
Idioma:
En
Revista:
Microbiol Immunol
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Australia