Dinucleotide cap analogue affinity resins for purification of proteins that specifically recognize the 5' end of mRNA.
Bioorg Med Chem Lett
; 21(20): 6131-4, 2011 Oct 15.
Article
en En
| MEDLINE
| ID: mdl-21889340
Here we present first dinucleotide affinity resins for purification of proteins that specifically recognize the 5' end of mRNA. Constructed resins possess either a naturally occurring mono- or trimethylated cap or their analogues resistant towards enzymatic degradation, bearing a CH(2) bridge between ß and γ position of the 5',5'-triphosphate chain. All cap analogues were attached to a polymer support (EAH-Sepharose) through the carboxylic group that had been generated by derivatization of the 2',3'-cis diol of the second nucleotide in the cap structure with levulinic acid.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Análogos de Caperuza de ARN
/
Caperuzas de ARN
/
Cromatografía de Afinidad
/
Proteínas de Caenorhabditis elegans
Límite:
Animals
Idioma:
En
Revista:
Bioorg Med Chem Lett
Asunto de la revista:
BIOQUIMICA
/
QUIMICA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Polonia
Pais de publicación:
Reino Unido