Cullin-5 and cullin-2 play a role in the development of neuromuscular junction and the female germ line of Drosophila.
J Genet
; 90(2): 239-49, 2011 Aug.
Article
en En
| MEDLINE
| ID: mdl-21869472
Cullins confer substrate specificity to E3-ligases which are multi-protein complexes involved in ubiquitin-mediated protein degradation or modification. There are six cullin genes in Drosophila melanogaster. We have raised an antibody against Cul-5 and demonstrated that it expresses in neuronal and non-neuronal cells throughout development. In the embryonic tracheal system, Cul-5 is enriched at fusion sites together with E-Cadherin and Fasciclin III. Mutations of cul-5 do not affect tracheal development but do show defects in the organization of synaptic boutons at the larval neuromuscular junction where the protein is expressed in a subset of motoneuron terminals. Loss of function of another cullin gene 'cul-2' results in similar defects at the larval neuromuscular junction although cul-2;cul-5 double mutants do not show an enhanced phenotype. Both cul-2 and cul-5 mutants show similar aberrations in the development of female germ line. Our results suggest that both of these cullin proteins participate in similar developmental processes.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Ovario
/
Proteínas de Drosophila
/
Proteínas Cullin
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Drosophila melanogaster
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Larva
/
Unión Neuromuscular
Límite:
Animals
Idioma:
En
Revista:
J Genet
Año:
2011
Tipo del documento:
Article
País de afiliación:
India
Pais de publicación:
India