In vitro suppression of UAG and UGA mutants in the thymidine kinase gene of herpes simplex virus.
Proc Natl Acad Sci U S A
; 76(1): 430-4, 1979 Jan.
Article
en En
| MEDLINE
| ID: mdl-218201
We have studied the in vitro translation, in nuclease-treated reticulocyte lysates, of mRNA from cells infected with several thymidine kinase-deficient mutants of herpes simplex virus type I. The addition of suppressor tRNAs from yeast resulted in suppression of the mutant property in the case of two mutants. Synthesis of enzymatically active viral thymidine kinase was restored by serine-inserting amber suppressor tRNA in the case of HSV TK4- and synthesis of the intact, but inactive, thymidine kinase protein was restored by serine- and leucine-inserting UGA suppressor tRNAs in the case of HSV TK43-. Read-through of the normal termination at the end of the thymidine kinase gene was promoted by UGA suppressor tRNAs. We conclude that HSV-TK4- is an amber (UAG) mutant and that HSV-TK43- is an opal (UGA) mutant.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Supresión Genética
/
Timidina Quinasa
/
Simplexvirus
/
Genes
Idioma:
En
Revista:
Proc Natl Acad Sci U S A
Año:
1979
Tipo del documento:
Article
Pais de publicación:
Estados Unidos