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Hypothesis-driven weight of evidence framework for evaluating data within the US EPA's Endocrine Disruptor Screening Program.
Borgert, Christopher J; Mihaich, Ellen M; Ortego, Lisa S; Bentley, Karin S; Holmes, Catherine M; Levine, Steven L; Becker, Richard A.
Afiliación
  • Borgert CJ; Applied Pharmacology & Toxicology, Inc., Gainesville, FL, USA. cjborgert@apt-pharmatox.com
Regul Toxicol Pharmacol ; 61(2): 185-91, 2011 Nov.
Article en En | MEDLINE | ID: mdl-21803110
"Weight of Evidence" (WoE) approaches are often used to critically examine, prioritize, and integrate results from different types of studies to reach general conclusions. For assessing hormonally active agents, WoE evaluations are necessary to assess screening assays that identify potential interactions with components of the endocrine system, long-term reproductive and developmental toxicity tests that define adverse effects, mode of action studies aimed at identifying toxicological pathways underlying adverse effects, and toxicity, exposure and pharmacokinetic data to characterize potential risks. We describe a hypothesis-driven WoE approach for hormonally active agents and illustrate the approach by constructing hypotheses for testing the premise that a substance interacts as an agonist or antagonist with components of estrogen, androgen, or thyroid pathways or with components of the aromatase or steroidogenic enzyme systems for evaluating data within the US EPA's Endocrine Disruptor Screening Program. Published recommendations are used to evaluate data validity for testing each hypothesis and quantitative weightings are proposed to reflect two data parameters. Relevance weightings should be derived for each endpoint to reflect the degree to which it probes each specific hypothesis. Response weightings should be derived based on assay results from the test substance compared to the range of responses produced in the assay by the appropriate prototype hormone and positive and negative controls. Overall WoE scores should be derived based on response and relevance weightings and a WoE narrative developed to clearly describe the final determinations.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema Endocrino / Disruptores Endocrinos Tipo de estudio: Diagnostic_studies / Etiology_studies / Evaluation_studies / Prognostic_studies / Screening_studies Límite: Animals / Humans País/Región como asunto: America do norte Idioma: En Revista: Regul Toxicol Pharmacol Año: 2011 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sistema Endocrino / Disruptores Endocrinos Tipo de estudio: Diagnostic_studies / Etiology_studies / Evaluation_studies / Prognostic_studies / Screening_studies Límite: Animals / Humans País/Región como asunto: America do norte Idioma: En Revista: Regul Toxicol Pharmacol Año: 2011 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Países Bajos