Prolonged storage of epididymal spermatozoa does not affect their capacity to fertilise in vitro-matured domestic cat (Felis catus) oocytes when using ICSI.
Reprod Fertil Dev
; 23(6): 818-25, 2011.
Article
en En
| MEDLINE
| ID: mdl-21791183
The impact of different storage conditions of epididymal spermatozoa (including prolonged storage, cryopreservation and freeze-drying) on their fertilisation capacity was tested using intracytoplasmic sperm injection (ICSI). This kind of information is urgently needed when applying assisted reproductive technology to endangered felids in zoos. In particular, the utilisation of epididymal spermatozoa of castrated or deceased felids often requires time-consuming transportation and is therefore susceptible to loss of gamete quality. Sperm cells were stored at 4 °C for up to 72 h followed by cryopreservation or freeze-drying. Thawed motile and immotile spermatozoa were used for ICSI and the embryo cleavage rate was assessed 36 h after injection. A significant impact on the fertilisation rate of oocytes could only be detected when using immotile thawed or rehydrated spermatozoa. Cryopreservation or storage at 4 °C showed no influence. The simulation of transport conditions using domestic cat spermatozoa revealed that in vitro production of felid embryos with gametes from euthanised individuals is possible if testes are stored cool and arrive at the laboratory within 72 h. An essential prerequisite is the application of ICSI to achieve fertilisation even with single motile spermatozoa. Additional cryopreservation of spermatozoa after transportation is possible and will allow the establishment of a sperm bank for felids.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Oocitos
/
Manejo de Especímenes
/
Fertilización In Vitro
/
Gatos
/
Criopreservación
/
Inyecciones de Esperma Intracitoplasmáticas
/
Recuperación de la Esperma
/
Fertilización
Tipo de estudio:
Evaluation_studies
Límite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Reprod Fertil Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Australia