Detection and phylogenetic analysis of peste des petits ruminants virus isolated from outbreaks in Punjab, Pakistan.
Transbound Emerg Dis
; 59(1): 85-93, 2012 Feb.
Article
en En
| MEDLINE
| ID: mdl-21777402
Peste des Petits Ruminants (PPR) is an important viral disease of small ruminants and is endemic in Pakistan. In the following study, samples from two outbreaks of PPR in goats have been subjected to laboratory investigations. The Peste des Petits Ruminants virus (PPRV) genome was detected using both conventional and real-time PCR. Genetic characterization of the local PPRV field isolates was conducted by sequencing 322 bp of the fusion (F) gene and 255 bp of the nucleoprotein (N) gene. The phylogenetic tree based on the F gene clustered samples from both outbreaks into lineage 4 along with other Asian isolates, specifically into subcluster 1 along with isolates from Middle East. Analysis of N gene revealed a different pattern. In this case, the Pakistani samples clustered with Chinese, Tajikistani and Iranian isolates, which probably represents the true geographical pattern of virus circulation. This is the first report presenting the phylogenetic tree based on N gene as well as performing a parallel comparison of the trees of F and N gene together from Pakistani isolates. The results of this study shed light on the PPRV population in Pakistan and emphasize the importance of using molecular methods to understand the epidemiology. Such understanding is essential in any efforts to control the number and impact of outbreaks that are occurring in endemic countries such as Pakistan, especially in the current scenario where OIE and FAO are eager to control and subsequently eradicate PPR from the globe, as has been achieved for Rinderpest.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Filogenia
/
Enfermedades de las Cabras
/
Brotes de Enfermedades
/
Virus de la Peste de los Pequeños Rumiantes
/
Peste de los Pequeños Rumiantes
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Transbound Emerg Dis
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Alemania