Neurokinin A induces expression of the c-fos, c-jun, and c-myc genes in rat smooth muscle cells.
Eur J Biochem
; 194(2): 527-32, 1990 Dec 12.
Article
en En
| MEDLINE
| ID: mdl-2176599
Neurokinin A, a member of the tachykinin family of neuropeptides, has been identified as a mitogen for cultured smooth muscle cells. Tachykinin-induced DNA synthesis has previously been shown to be mediated by a receptor-specific mechanism and to correlate with accumulation of phosphatidylinositol 4,5-bisphosphate breakdown products. In the present experiments, we have studied intracellular pH and expression of the proto-oncogenes c-myc, c-jun and c-fos in smooth muscle cells exposed to mitogenic concentrations of neurokinin A. Growth-arrested smooth muscle cells stimulated with neurokinin A responded with an amiloride-sensitive intracellular alkalinization, indicating Na+/H+ antiport activation. c-myc and c-jun mRNA expression was only slightly elevated by neurokinin A, while c-fos expression underwent a more pronounced increase. Maximal levels of c-fos transcripts were found after 15 min and 30 min following neurokinin A stimulation. The results demonstrate that neuropeptides may influence proto-oncogene expression in smooth muscle cells and suggest a mechanism by which peripheral neurons may modulate differentiation and growth of these cells.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Factores de Transcripción
/
Proto-Oncogenes
/
Neuroquinina A
/
Regulación de la Expresión Génica
/
Proteínas Proto-Oncogénicas c-myc
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Proteínas Proto-Oncogénicas
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Proteínas de Unión al ADN
/
Músculo Liso
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Eur J Biochem
Año:
1990
Tipo del documento:
Article
País de afiliación:
Suecia
Pais de publicación:
Reino Unido