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Sensitivity to lovastatin of Saccharomyces cerevisiae strains deleted for pleiotropic drug resistance (PDR) genes.
Formenti, Luca Riccardo; Kielland-Brandt, Morten C.
Afiliación
  • Formenti LR; Department of Systems Biology, Center for Microbial Biotechnology, Technical University of Denmark, Lyngby. lrf @ bio.dtu.dk
J Mol Microbiol Biotechnol ; 20(4): 191-5, 2011.
Article en En | MEDLINE | ID: mdl-21757925
The use of statins is well established in human therapy, and model organisms such as Saccharomyces cerevisiae are commonly used in studies of drug action at molecular and cellular levels. The investigation of the resistance mechanisms towards statins may suggest new approaches to improve therapy based on the use of statins. We investigated the susceptibility to lovastatin of S. cerevisiae strains deleted for PDR genes, responsible for exporting hydrophobic and amphiphilic drugs, such as lovastatin. Strains deleted for the genes tested, PDR1, PDR3, PDR5 and SNQ2, exhibited remarkably different phenotypes, with deletion of PDR5 causing the highest sensitivity to lovastatin. The study helped clarifying which pdr mutants to use in studies of physiological actions of statins in yeast.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lovastatina / Eliminación de Gen / Transportadoras de Casetes de Unión a ATP / Proteínas de Saccharomyces cerevisiae / Farmacorresistencia Fúngica / Genes Fúngicos / Anticolesterolemiantes Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Mol Microbiol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA / MICROBIOLOGIA Año: 2011 Tipo del documento: Article Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Lovastatina / Eliminación de Gen / Transportadoras de Casetes de Unión a ATP / Proteínas de Saccharomyces cerevisiae / Farmacorresistencia Fúngica / Genes Fúngicos / Anticolesterolemiantes Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: J Mol Microbiol Biotechnol Asunto de la revista: BIOLOGIA MOLECULAR / BIOTECNOLOGIA / MICROBIOLOGIA Año: 2011 Tipo del documento: Article Pais de publicación: Suiza