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Cyclosporin A inhibits the replication of diverse coronaviruses.
de Wilde, Adriaan H; Zevenhoven-Dobbe, Jessika C; van der Meer, Yvonne; Thiel, Volker; Narayanan, Krishna; Makino, Shinji; Snijder, Eric J; van Hemert, Martijn J.
Afiliación
  • de Wilde AH; Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • Zevenhoven-Dobbe JC; Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • van der Meer Y; Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
  • Thiel V; Vetsuisse Faculty, University of Zürich, Zürich, Switzerland.
  • Narayanan K; Institute of Immunobiology, Kantonal Hospital St Gallen, St Gallen, Switzerland.
  • Makino S; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
  • Snijder EJ; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA.
  • van Hemert MJ; Molecular Virology Laboratory, Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands.
J Gen Virol ; 92(Pt 11): 2542-2548, 2011 Nov.
Article en En | MEDLINE | ID: mdl-21752960
Low micromolar, non-cytotoxic concentrations of cyclosporin A (CsA) strongly affected the replication of severe acute respiratory syndrome coronavirus (SARS-CoV), human coronavirus 229E and mouse hepatitis virus in cell culture, as was evident from the strong inhibition of GFP reporter gene expression and a reduction of up to 4 logs in progeny titres. Upon high-multiplicity infection, CsA treatment rendered SARS-CoV RNA and protein synthesis almost undetectable, suggesting an early block in replication. siRNA-mediated knockdown of the expression of the prominent CsA targets cyclophilin A and B did not affect SARS-CoV replication, suggesting either that these specific cyclophilin family members are dispensable or that the reduced expression levels suffice to support replication.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Ciclosporina / Virus de la Hepatitis Murina / Coronavirus Humano 229E / Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2011 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antivirales / Ciclosporina / Virus de la Hepatitis Murina / Coronavirus Humano 229E / Coronavirus Relacionado al Síndrome Respiratorio Agudo Severo Límite: Animals / Humans Idioma: En Revista: J Gen Virol Año: 2011 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido