Preparation and characterisation of in-house reference material of tylosin in honey and results of a proficiency test.
Anal Chim Acta
; 700(1-2): 58-62, 2011 Aug 26.
Article
en En
| MEDLINE
| ID: mdl-21742117
The analysis of incurred material from animals treated with pharmacologically active substances is an efficient way to check the accuracy of a method. Tylosin A was chosen for the preparation of that material because it is highly effective in controlling active infections of American Foulbrood (AFB), a global threat to apiculture, but residues in honey are not allowed according to European legislation. For this reason an in-house reference material of honey containing the macrolide tylosin A and its degradation product desmycosin (tylosin B) was prepared. After the treatment of a beehive with the appropriate macrolide tylosin A, the honey samples were collected. The incurred honey material was diluted by mixing with blank honey. Concentrations of 25.81 µg kg(-1) for tylosin A and of 19.28 µg kg(-1) for its degradation product desmycosin (tylosin B) were reached. The homogeneity was checked by analysing 12 bottles in duplicate. The stability was tested at different defined temperatures and storage conditions. The reference material described above was homogeneous and stable. Samples of this in-house reference material were used for the realisation of a proficiency test with international participation. All participants accomplished satisfying results with the exception of one laboratory.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Tilosina
/
Cromatografía Líquida de Alta Presión
/
Espectrometría de Masas en Tándem
/
Miel
/
Antibacterianos
Límite:
Animals
Idioma:
En
Revista:
Anal Chim Acta
Año:
2011
Tipo del documento:
Article
País de afiliación:
Alemania
Pais de publicación:
Países Bajos