Cell cytoskeleton and tether extraction.
Biophys J
; 101(1): 43-52, 2011 Jul 06.
Article
en En
| MEDLINE
| ID: mdl-21723813
We perform a detailed investigation of the force × deformation curve in tether extraction from 3T3 cells by optical tweezers. Contrary to conventional wisdom about tethers extracted from cells, we find that actin filaments are present within them, so that a revised theory of tether pulling from cells is called for. We also measure steady and maximum tether force values significantly higher than previously published ones for 3T3 cells. Possible explanations for these differences are investigated. Further experimental support of the theory of force barriers for membrane tube extension is obtained. The potential of studies on tether pulling force × deformation for retrieving information on membrane-cytoskeleton interaction is emphasized.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Citoesqueleto
/
Fibroblastos
Límite:
Animals
Idioma:
En
Revista:
Biophys J
Año:
2011
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos