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Monocytic microparticles activate endothelial cells in an IL-1ß-dependent manner.
Wang, Jian-Guo; Williams, Julie C; Davis, Beckley K; Jacobson, Ken; Doerschuk, Claire M; Ting, Jenny P-Y; Mackman, Nigel.
Afiliación
  • Wang JG; Division of Hematology and Oncology, Department of Medicine, McAllister Heart Institute, Chapel Hill, NC, USA.
Blood ; 118(8): 2366-74, 2011 Aug 25.
Article en En | MEDLINE | ID: mdl-21700772
Microparticles (MPs) are shed from activated and dying cells. They can transmit signals from cell to cell, locally or at a distance through the circulation. Monocytic MPs are elevated in different diseases, including bacterial infections. Here, we investigated how monocytic MPs activate endothelial cells. We found that MPs from lipopolysaccharide (LPS)-treated THP-1 monocytic cells bind to and are internalized by human endothelial cells. MPs from LPS-treated THP-1 cells, but not untreated cells, induced phosphorylation of ERK1/2, activation of the nuclear factor-κB pathway and expression of cell adhesion molecules intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin. Similar results were observed using MPs from LPS-treated peripheral blood mononuclear cells. We next investigated the mechanism by which monocytic MPs activated endothelial cells and found that they contain IL-1ß and components of the inflammasome, including apoptosis-associated speck-like protein containing a CARD, caspase-1, and NLRP3. Importantly, knockdown of NLRP3 in THP-1 cells reduced the activity of the MPs and blockade of the IL-1 receptor on endothelial cells decreased MP-dependent induction of cell adhesion molecules. Therefore, monocytic MPs contain IL-1ß and may amplify inflammation by enhancing the activation of the endothelium.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monocitos / Células Endoteliales / Interleucina-1beta / Micropartículas Derivadas de Células Límite: Humans Idioma: En Revista: Blood Año: 2011 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Monocitos / Células Endoteliales / Interleucina-1beta / Micropartículas Derivadas de Células Límite: Humans Idioma: En Revista: Blood Año: 2011 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos