Characterization of vasoactive intestinal peptide receptors in retina.
Exp Eye Res
; 51(3): 317-23, 1990 Sep.
Article
en En
| MEDLINE
| ID: mdl-2169429
Vasoactive intestinal peptide (VIP) is a neuropeptide having a wide range of effects on a large number of tissues. To gain insight into the role VIP plays in retinal function, VIP receptors in bovine retinal membranes were analyzed in competition binding assays and by affinity labeling studies and compared to VIP receptors in rat liver membranes. In both membrane preparations, high affinity VIP binding sites (KD approximately 1 nM) were detected. Secretin and glucagon, each having close structural homology to VIP, were found to have negligible effects on [125I]VIP binding in retina. In contrast, secretin (KD = 70 nM) was modestly effective in inhibiting [125I]VIP binding to rat liver membranes. Affinity labeling analysis revealed a VIP binding site of 59 kDa in both bovine retinal and rat liver membranes. Digestion of affinity-labeled receptor proteins with endoglycosidase F generated final cleavage products of approx. 45 kDa for both receptors. These results indicate that the retina expresses a high affinity, highly selective VIP receptor thereby supporting a specific function for VIP in this tissue.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Retina
/
Receptores de la Hormona Gastrointestinal
/
Péptido Intestinal Vasoactivo
Límite:
Animals
Idioma:
En
Revista:
Exp Eye Res
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Reino Unido