Nab2 functions in the metabolism of RNA driven by polymerases II and III.
Mol Biol Cell
; 22(15): 2729-40, 2011 Aug 01.
Article
en En
| MEDLINE
| ID: mdl-21680710
Gene expression in eukaryotes is an essential process that includes transcription, RNA processing, and export. One important player in this interface is the poly(A)(+)-RNA-binding protein Nab2, which regulates the mRNA poly(A)(+)-tail length and export. Here we show that Nab2 has additional roles during mRNA transcription, tRNA metabolism, and ribosomal subunit export. Nab2 is associated with the entire open reading frame of actively transcribed RNA polymerase (RNAP) II and III genes. As a consequence, nab2 mutations confer translation defects that are detected by polysome profiling. Genome-wide analysis of expression of a conditional degron nab2 mutant shows that the role of Nab2 in RNAPII transcription and RNAPIII metabolism is direct. Taken together, our results identify novel functions for Nab2 in transcription and metabolism of most types of RNAs, indicating that Nab2 function is more ubiquitous than previously anticipated, and that it is a central player in the general and coordinated control of gene expression from transcription to translation.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Poli A
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Polirribosomas
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Saccharomyces cerevisiae
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ARN Polimerasa II
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ARN Polimerasa III
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Regulación Fúngica de la Expresión Génica
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Proteínas de Unión al ARN
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Proteínas de Transporte Nucleocitoplasmático
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Revista:
Mol Biol Cell
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2011
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Estados Unidos