Inhibition effect of Gynura procumbens extract on UV-B-induced matrix-metalloproteinase expression in human dermal fibroblasts.

Kim, Junoh; Lee, Chan-Woo; Kim, Eun Kyung; Lee, Su-Jin; Park, Nok-Hyun; Kim, Han-Sung; Kim, Han-Kon; Char, Kookheon; Jang, Young Pyo; Kim, Jin-Woong

Kim, Junoh; Lee, Chan-Woo; Kim, Eun Kyung; Lee, Su-Jin; Park, Nok-Hyun; Kim, Han-Sung; Kim, Han-Kon; Char, Kookheon; Jang, Young Pyo; Kim, Jin-Woong.

Afiliación

Kim J; Amore-Pacific Co. R&D Center, 314-1, Bora-dong, Giheung-gu, Yongin-si, Gyeonggi-Do 446-729, Republic of Korea.

J Ethnopharmacol ; 137(1): 427-33, 2011 Sep 01.

Article en En | MEDLINE | ID: mdl-21672616

RESUMEN

ETHNOPHARMACOLOGICAL RELEVANCE: Gynura procumbens Merr. (Asteraceae) has been used as a traditional remedy for various skin diseases in certain areas of Southeast Asia. AIM OF THE STUDY: In order to evaluate the protective activity of Gynura procumbens extract on skin photoaging and elucidate its mode of action. MATERIALS AND METHODS: Matrix-metalloproteinase (MMP)-1 and -9 expressions were induced by UV-B irradiation in human primary dermal fibroblasts. MMP-1 expression level was measured by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis. Zymography was employed for evaluating the enzymatic activity of MMP-9. Anti-inflammatory activity and anti-oxidative capacity of the extract were evaluated by ELISA and dichlorodihydrofluorescein diacetate (DCF-DA) assay. RESULTS: The ethanolic extract of Gynura procumbens inhibited MMP-1 expression up to 70% compare to negative control group. The enzymatic activity of MMP-9 was inhibited around 73% by the treatment of 20µg/mL of the extract. The extract markedly reduced the production of reactive oxygen species (ROS). Gynura procumbens extract showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in human HaCat keratinocyte. CONCLUSION: The ethanolic extract of Gynura procumbens inhibited MMP-1 and MMP-9 expressions induced by UV-B irradiation via inhibition of pro-inflammatory cytokine mediator release and ROS production.

Asunto(s)

Asteraceae; Fármacos Dermatológicos/farmacología; Dermis/efectos de los fármacos; Fibroblastos/efectos de los fármacos; Metaloproteinasa 1 de la Matriz/metabolismo; Metaloproteinasa 9 de la Matriz/metabolismo; Extractos Vegetales/farmacología; Envejecimiento de la Piel/efectos de los fármacos; Rayos Ultravioleta; Antiinflamatorios/farmacología; Antioxidantes/farmacología; Asteraceae/química; Western Blotting; Células Cultivadas; Cromatografía Líquida de Alta Presión; Fármacos Dermatológicos/química; Fármacos Dermatológicos/aislamiento & purificación; Dermis/enzimología; Dermis/efectos de la radiación; Relación Dosis-Respuesta a Droga; Ensayo de Inmunoadsorción Enzimática; Etanol/química; Fibroblastos/enzimología; Fibroblastos/efectos de la radiación; Humanos; Mediadores de Inflamación/metabolismo; Interleucina-6/metabolismo; Interleucina-8/metabolismo; Extractos Vegetales/química; Extractos Vegetales/aislamiento & purificación; Hojas de la Planta; Especies Reactivas de Oxígeno/metabolismo; Envejecimiento de la Piel/efectos de la radiación; Solventes/química

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Rayos Ultravioleta / Extractos Vegetales / Envejecimiento de la Piel / Asteraceae / Dermis / Metaloproteinasa 9 de la Matriz / Metaloproteinasa 1 de la Matriz / Fármacos Dermatológicos / Fibroblastos Límite: Humans Idioma: En Revista: J Ethnopharmacol Año: 2011 Tipo del documento: Article Pais de publicación: Irlanda

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