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Complete blood count using VCS (volume, conductivity, light scatter) technology is affected by hyperlipidemia in a child with acute leukemia.
Gokcebay, D G; Azik, F M; Isik, P; Bozkaya, I O; Kara, A; Tavil, E B; Yarali, N; Tunc, B.
Afiliación
  • Gokcebay DG; Department of Pediatric Hematology, Ankara Children's Hematology and Oncology Hospital, Ankara, Turkey. drdilekgurlek@hotmail.com
Int J Lab Hematol ; 33(6): 651-5, 2011 Dec.
Article en En | MEDLINE | ID: mdl-21645281
Asparaginase, an effective drug in the treatment of childhood acute lymphoblastic leukemia (ALL), has become an important component of most childhood ALL regimens during the remission induction or intensification phases of treatment. The incidence range of asparaginase-associated lipid abnormalities that are seen in children is 67-72%. Lipemia causes erroneous results, which uses photometric methods to analyze blood samples. We describe a case of l-asparaginase-associated severe hyperlipidemia with complete blood count abnormalities. Complete blood count analysis was performed with Beckman COULTER(®) GEN·S™ system, which uses the Coulter Volume, Conductivity, Scatter technology to probe hydrodynamically focused cells. Although an expected significant inaccuracy in hemoglobin determination occurred starting from a lipid value of 3450 mg/dl, we observed that triglyceride level was 1466 mg/dl. Complete blood count analysis revealed that exceptionally high hemoglobin, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration levels vs. discordant with red blood cell count, mean corpuscular volume, and hematocrit levels. Total leukocyte count altered spontaneously in a wide range, and was checked with blood smear. Platelet count was in expected range (Table 1). Thus, we thought it was a laboratory error, and the patient's follow-up especially for red cell parameters was made by red blood cell and hematocrit values.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Laboratorio Clínico / Leucemia-Linfoma Linfoblástico de Células Precursoras / Pruebas Hematológicas / Hiperlipidemias Tipo de estudio: Diagnostic_studies Límite: Child, preschool / Female / Humans Idioma: En Revista: Int J Lab Hematol Asunto de la revista: HEMATOLOGIA Año: 2011 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Laboratorio Clínico / Leucemia-Linfoma Linfoblástico de Células Precursoras / Pruebas Hematológicas / Hiperlipidemias Tipo de estudio: Diagnostic_studies Límite: Child, preschool / Female / Humans Idioma: En Revista: Int J Lab Hematol Asunto de la revista: HEMATOLOGIA Año: 2011 Tipo del documento: Article País de afiliación: Turquía Pais de publicación: Reino Unido