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High-fat feeding promotes obesity via insulin receptor/PI3K-dependent inhibition of SF-1 VMH neurons.
Nat Neurosci ; 14(7): 911-8, 2011 Jun 05.
Article en En | MEDLINE | ID: mdl-21642975
Steroidogenic factor 1 (SF-1)-expressing neurons of the ventromedial hypothalamus (VMH) control energy homeostasis, but the role of insulin action in these cells remains undefined. We show that insulin activates phosphatidylinositol-3-OH kinase (PI3K) signaling in SF-1 neurons and reduces firing frequency in these cells through activation of K(ATP) channels. These effects were abrogated in mice with insulin receptor deficiency restricted to SF-1 neurons (SF-1(ΔIR) mice). Whereas body weight and glucose homeostasis remained the same in SF-1(ΔIR) mice as in controls under a normal chow diet, they were protected from diet-induced leptin resistance, weight gain, adiposity and impaired glucose tolerance. High-fat feeding activated PI3K signaling in SF-1 neurons of control mice, and this response was attenuated in the VMH of SF-1(ΔIR) mice. Mimicking diet-induced overactivation of PI3K signaling by disruption of the phosphatidylinositol-3,4,5-trisphosphate phosphatase PTEN led to increased body weight and hyperphagia under a normal chow diet. Collectively, our experiments reveal that high-fat diet-induced, insulin-dependent PI3K activation in VMH neurons contributes to obesity development.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Hipotalámico Ventromedial / Receptor de Insulina / Grasas de la Dieta / Fosfatidilinositol 3-Quinasas / Neuronas / Obesidad Idioma: En Revista: Nat Neurosci Asunto de la revista: NEUROLOGIA Año: 2011 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Núcleo Hipotalámico Ventromedial / Receptor de Insulina / Grasas de la Dieta / Fosfatidilinositol 3-Quinasas / Neuronas / Obesidad Idioma: En Revista: Nat Neurosci Asunto de la revista: NEUROLOGIA Año: 2011 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos