The evaluation of the genotoxic and oxidative damage potentials of Ulothrix tenuissima (Kutz.) in vitro.
Toxicol Ind Health
; 28(2): 147-51, 2012 Mar.
Article
en En
| MEDLINE
| ID: mdl-21632573
Several alga species are known to produce a variety of toxic metabolites that pose a threat to aquatic organisms, animals and humans. Moreover, these metabolites have been thought to cause serious diseases including certain cancers and neurodegenerative disorders. On the other hand, Ulothrix is a genus of filamentous green algae, generally found in fresh water and marine and abundantly available in some lakes and rivers of Turkey. To our best knowledge, no study has been performed to assess the genotoxic and biochemical effects of U. tenuissima on cultured human blood cells. Therefore, in order to determine clastogenic or aneugenic effects of aqueous alga extracts the micronucleus assay was carried out. Nuclear division index (NDI) in peripheral lymphocytes was also analyzed for cytotoxicity evaluations. In addition, biochemical parameters (total antioxidant capacity (TAC) and total oxidative stress (TOS)) were examined to determine oxidative effects. For this aim, we obtained heparinized blood samples from three healthy persons. The alga samples were collected from Porsuk Pond in Hasankale (Erzurum, Turkey) in summer period of the year 2010. The aqueous extracts of this species were added to cultures at different concentrations (0 to 5000 ppm) for 72 h. Our results showed that this alga did not cause any statistically important changes in the rates of studied genotoxicity endpoint. But dose-dependent alterations were observed in TAC and TOS levels and NDI rates. In conclusion, U. tenuissima was found to be non-genotoxic but caused sterility at higher concentrations due to oxidative stress.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Estrés Oxidativo
/
Micronúcleos con Defecto Cromosómico
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Chlorophyta
Límite:
Adult
/
Humans
País/Región como asunto:
Asia
Idioma:
En
Revista:
Toxicol Ind Health
Asunto de la revista:
MEDICINA OCUPACIONAL
/
TOXICOLOGIA
Año:
2012
Tipo del documento:
Article
País de afiliación:
Turquía
Pais de publicación:
Reino Unido