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A protein-based pneumococcal vaccine protects rhesus macaques from pneumonia after experimental infection with Streptococcus pneumoniae.
Denoël, Philippe; Philipp, Mario T; Doyle, Lara; Martin, Dale; Carletti, Georges; Poolman, Jan T.
Afiliación
  • Denoël P; GlaxoSmithKline Biologicals, Rixensart, Belgium.
Vaccine ; 29(33): 5495-501, 2011 Jul 26.
Article en En | MEDLINE | ID: mdl-21624422
Infections caused by Streptococcus pneumoniae are a major cause of mortality throughout the world. Protein-based pneumococcal vaccines are envisaged to replace or complement the current polysaccharide-based vaccines. In this context, detoxified pneumolysin (dPly) and pneumococcal histidine triad protein D (PhtD) are two potential candidates for incorporation into pneumococcal vaccines. In this study, the protective efficacy of a PhtD-dPly vaccine was evaluated in a rhesus macaque (Macaca mulatta) model of pneumonia. The animals were immunized twice with 10 µg of PhtD and 10 µg of dPly formulated in the Adjuvant System AS02 or with AS02 alone, before they were challenged with a 19F pneumococcal strain. The survival was significantly higher in the protein-vaccinated group and seemed to be linked to the capacity to greatly reduce bacterial load within the first week post-challenge. Vaccination elicited high concentrations of anti-PhtD and anti-Ply antibodies and a link was found between survival and antibody levels. In conclusion, AS02-adjuvanted PhtD-dPly vaccine protects against S. pneumoniae-induced pneumonia. It is probable that the protection is at least partially mediated by PhtD- and Ply-specific antibodies.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Neumocócica / Streptococcus pneumoniae / Vacunas Neumococicas / Antígenos Bacterianos Límite: Animals Idioma: En Revista: Vaccine Año: 2011 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Países Bajos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neumonía Neumocócica / Streptococcus pneumoniae / Vacunas Neumococicas / Antígenos Bacterianos Límite: Animals Idioma: En Revista: Vaccine Año: 2011 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Países Bajos