In vitro evaluation of the microbial contamination on new toothbrushes: a preliminary study.
Microsc Res Tech
; 75(1): 42-5, 2012 Jan.
Article
en En
| MEDLINE
| ID: mdl-21618654
OBJECTIVE: The presence and survival of microorganisms on toothbrush bristles might play a role on the etiology of oral infections. The aim of this in vitro study was to evaluate the presence of bacterial contamination on new toothbrushes before oral contact. MATERIALS AND METHODS: Forty toothbrushes from five different manufacturers were used in this experimental study. Each manufacturer was divided according to conventional local of obtaining: industry, drugstore, market, and perfumery. The toothbrush heads were completely immersed into tubes containing 5.0 mL of sterile peptonated water (dilution 1:10). A group of eight tubes containing the sterile solution was used as control. After 21 days of anaerobic incubation, occurrence of contamination was visually evaluated and confirmed by light microscopy. RESULTS: Bacterial growth in the medium, indicative of bristles contamination, was found in a total of 19 out of 40 samples (47.5%) evaluated: 6 out of 14 samples (42.85%) from industry group, 4 out of 8 samples (50.0%) from drugstore, 5 out of 10 samples (50.0%) from market, and 4 out of 8 samples (50.0%) from perfumery. Only the toothbrushes with bristles coated with chlorhexidine did not show contamination. The Gram-negative sporulating bacilli were the most prevalent form recovered. CONCLUSIONS: Except for chlorhexidine group, bacterial growth was observed in all groups evaluated irrespective local of obtaining.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Bacterias
/
Cepillado Dental
/
Contaminación de Equipos
/
Seguridad de Productos para el Consumidor
/
Productos Domésticos
Idioma:
En
Revista:
Microsc Res Tech
Asunto de la revista:
DIAGNOSTICO POR IMAGEM
Año:
2012
Tipo del documento:
Article
País de afiliación:
Brasil
Pais de publicación:
Estados Unidos