A sensitive and specific assay for superoxide anion released by neutrophils or macrophages based on bioluminescence of polynoidin.
Anal Biochem
; 184(2): 369-74, 1990 Feb 01.
Article
en En
| MEDLINE
| ID: mdl-2158250
Using the luminescent protein polynoidin, present in the bioluminescent system isolated from the marine annelid Harmothoe lunulata, we have developed a new method to measure, specifically, superoxide anion (O2-) released by macrophages or neutrophils. A small quantity of an aqueous crude extract of polynoidin is used to detect O2- released by stimulated cells. Light emission is linearly dependent on the number of cells over a wide range (10(3) to 10(7) cells), and the assay is thus more sensitive than either luminol or ferricytochrome c reduction. Luminescence is enhanced 20% by mannitol, 80% by catalase, and is totally quenched by superoxide dismutase. For the same number of cells, neutrophils showed a threefold higher release of O2- and a twofold faster first-order light decay than stimulated macrophages, in accordance with data obtained by other methods.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Superóxidos
/
Proteínas Luminiscentes
/
Macrófagos
/
Neutrófilos
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Anal Biochem
Año:
1990
Tipo del documento:
Article
Pais de publicación:
Estados Unidos