Potentiation of forskolin-induced increase of cAMP by diamide and N-ethylmaleimide in rat pancreatic islets.

Anazodo, M I; Müller, A B; Safayhi, H; Ammon, H P

Anazodo, M I; Müller, A B; Safayhi, H; Ammon, H P.

Afiliación

Anazodo MI; Pharmazeutisches Institut, Abteilung Pharmakologie, Universität Tübingen, Germany.

Horm Metab Res ; 22(2): 61-4, 1990 Feb.

Article en En | MEDLINE | ID: mdl-2157653

RESUMEN

In isolated rat pancreatic islets, the effect of diamide and N-ethylmaleimide (NEM) on forskolin- as well as on glucagon-induced elevation of cAMP was studied. Forskolin and glucagon increased cAMP levels in batch-incubated islets. Diamide and NEM further augmented forskolin-induced increase of cAMP levels, whereas glucagon-stimulated elevation of cAMP was not affected. From our data it is likely that under the conditions of the present study, the thiols related to the Ns-protein and the catalytic unit are insensitive to oxidation and alkylation. The potentiation of forskolin-induced cAMP production in intact islet cells by diamide and NEM may be due to inactivation of the thiols related to the Ni-protein.

Asunto(s)

Compuestos Azo/farmacología; Colforsina/farmacología; AMP Cíclico/biosíntesis; Diamida/farmacología; Etilmaleimida/farmacología; Islotes Pancreáticos/metabolismo; Animales; Sinergismo Farmacológico; Inducción Enzimática/efectos de los fármacos; Glucagón/farmacología; Ratas

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos Azo / Colforsina / Islotes Pancreáticos / AMP Cíclico / Diamida / Etilmaleimida Límite: Animals Idioma: En Revista: Horm Metab Res Año: 1990 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Alemania

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