Single-chain variable fragments antibody of CART inhibits the expression of cocaine-induced behavioral sensitization.
Neuroreport
; 22(9): 433-6, 2011 Jun 22.
Article
en En
| MEDLINE
| ID: mdl-21544003
Cocaine and amphetamine-regulated transcript (CART) peptides are neurotransmitters with important roles in drug abuse. The increase of CART expression in the brain induced by psychostimulants is associated with changes of behavior in addicted animals. We expressed and purified the single-chain variable fragments antibody (scFv) against CART55-102, and observed the effect of CART scFv on the expression of cocaine-induced behavior sensitization in mice. Results showed that the titer of CART scFv was 1.6 µg/ml. Single administration of CART scFv (intraperitoneal 0.04, 0.2, and 1 mg/kg) reduced the increasing locomotor activity induced by chronic cocaine intake in mice (P<0.05-0.01), but failed to affect the locomotor activity of naive mice. These results suggested that CART scFv may be a potential therapeutic tool to treat drug abuse.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Región Variable de Inmunoglobulina
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Trastornos Relacionados con Cocaína
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Anticuerpos de Cadena Única
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Actividad Motora
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Proteínas del Tejido Nervioso
Límite:
Animals
Idioma:
En
Revista:
Neuroreport
Asunto de la revista:
NEUROLOGIA
Año:
2011
Tipo del documento:
Article
Pais de publicación:
Reino Unido