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Ultrasound-guided serial transabdominal cardiac biopsies in cats.
van Essen, Vivian J; Uilenreef, Joost J; Szatmári, Viktor; Veldhuis Kroeze, Edwin J B; Kuiper, Raoul V; Rothuizen, Jan; de Bruin, Alain.
Afiliación
  • van Essen VJ; Department of Pathobiology, Faculty of Veterinary Medicine, Utrecht University, Yalelaan 1, 3584 CL Utrecht, The Netherlands.
Vet J ; 191(3): 341-6, 2012 Mar.
Article en En | MEDLINE | ID: mdl-21498093
A percutaneous/transdiaphragmatic core needle biopsy technique was developed in cats to obtain serial biopsies from different locations of the left ventricle, through which morphological and molecular changes within the same individual can be studied to unravel the mechanisms of feline cardiomyopathies. Transmural left ventricular myocardial samples were obtained from 29 anesthetized, healthy, adult cats with ultrasound guidance. An 18G automatic biopsy needle was inserted between the last left rib and the sternum through the diaphragm into the thorax. Biopsies were obtained from the left ventricular wall. In five cats, three single biopsies were taken with 4-week intervals. Autopsy was performed on six cats, of which three cats had serial biopsies. In total, 87 biopsies were obtained without long-term effects on cardiac function or structure. The biopsies caused transient single ventricular premature complexes and mild pericardial effusion without tamponade. Necropsy revealed a minimal amount of fibrous connective tissue in the diaphragm and the heart without any significant microscopic lesions in the adjacent muscle tissue. The high quality biopsy material was suitable for morphological and molecular studies. This minimally invasive, ultrasound-guided cardiac biopsy technique thus allows for the safe collection of serial biopsies to study feline cardiomyopathies in an experimental setting.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gatos / Ultrasonografía / Miocardio Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2012 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Gatos / Ultrasonografía / Miocardio Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Vet J Asunto de la revista: MEDICINA VETERINARIA Año: 2012 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Reino Unido