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[Expression of CD38 and HLA-DR on CD8+ T cells in pediatric AIDS patients receiving highly active antiretroviral therapy (HAART)].
Jin, Chang-Zhong; Feng, Lei; Xie, Tian-Sheng; Wu, Li-Juan; Fang, Mei-Xin; Zhang, Fu-Jie; Zhao, Yan; Wu, Nan-Ping.
Afiliación
  • Jin CZ; State Key Laboratory for Diagnosis and Treatment of Infectious Disease, First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310003, China.
Zhonghua Er Ke Za Zhi ; 49(1): 49-52, 2011 Jan.
Article en Zh | MEDLINE | ID: mdl-21429312
OBJECTIVE: To study the expression of CD38 and HLA-DR on CD8(+) T cells in pediatric AIDS patients receiving highly active antiretroviral therapy (HAART) and the relationship of immune activation and disease progression. METHODS: A cross-section study of 194 pediatric AIDS patients receiving HAART was carried out and 52 age-matched healthy children were recruited as control. The percentage of CD4(+), CD8(+), CD8(+)/CD38(+) and CD8(+)/HLA-DR(+) T cells was tested using flow cytometry, and HIV-RNA in plasma was detected by quantitative RT-PCR. RESULTS: One hundred and ninety-four pediatric AIDS patients were divided into two groups according to the viral load: 59 patients with VL ≥ 400 copies/ml and 135 patients with VL < 400 copies/ml. The percentage of CD8(+)/CD38(+) and CD8(+)/HLA-DR(+) T cells of patients with VL ≥ 400 copies/ml was significantly higher than that of patients with VL < 400 copies/ml (P < 0.05). Of patients with VL < 400 copies/ml, the percentage of CD8(+)/CD38(+) T cells was nearly normal, and the percentage of CD8(+)/HLA-DR(+) T cells was higher than normal level (P < 0.05). There was a positive correlation between percentage of CD8(+)/CD38(+) and of CD8(+)/HLA-DR(+)T cells and viral load (R = 0.403, P = 0.03 for the former and R = 0.569, P = 0.09 for the later). CONCLUSIONS: Effective HAART could decrease immune activation of HIV-infected children significantly. And there was a positive correlation between percentage of CD8(+)/CD38(+) and of CD8(+)/HLA-DR(+)T cells and viral load, suggesting that the two indicators might be used as the substitution of viral load in resource-limited areas.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos HLA-DR / Síndrome de Inmunodeficiencia Adquirida / Linfocitos T CD8-positivos / Terapia Antirretroviral Altamente Activa / ADP-Ribosil Ciclasa 1 Tipo de estudio: Observational_studies Límite: Adolescent / Child / Female / Humans / Male Idioma: Zh Revista: Zhonghua Er Ke Za Zhi Año: 2011 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Antígenos HLA-DR / Síndrome de Inmunodeficiencia Adquirida / Linfocitos T CD8-positivos / Terapia Antirretroviral Altamente Activa / ADP-Ribosil Ciclasa 1 Tipo de estudio: Observational_studies Límite: Adolescent / Child / Female / Humans / Male Idioma: Zh Revista: Zhonghua Er Ke Za Zhi Año: 2011 Tipo del documento: Article País de afiliación: China Pais de publicación: China