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Outbreak of Klebsiella pneumoniae carbapenemase 2-producing K. pneumoniae with high qnr prevalence in a Chinese hospital.
Zhang, Rong; Wang, Xuan Ding; Cai, Jia Chang; Zhou, Hong Wei; Lv, Huo Xiang; Hu, Qing Feng; Chen, Gong-Xiang.
Afiliación
  • Zhang R; 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
  • Wang XD; 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
  • Cai JC; 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
  • Zhou HW; 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
  • Lv HX; Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310009, PR China.
  • Hu QF; Zhejiang Provincial People's Hospital, 158 Shangtang Road, Hangzhou 310009, PR China.
  • Chen GX; 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Road, Hangzhou 310009, PR China.
J Med Microbiol ; 60(Pt 7): 977-982, 2011 Jul.
Article en En | MEDLINE | ID: mdl-21393450
Forty carbapenem-resistant Klebsiella pneumoniae isolates were recovered from 28 patients from various sites in an intensive care unit in Zhejiang Provincial People's Hospital, China, over a 6 month period. PFGE analysis indicated that the 40 strains were all closely related. The MICs of carbapenems varied from 16 to >256 µg ml⁻¹. Conjugation studies with Escherichia coli resulted in the transfer of reduced carbapenem susceptibility from the original isolates. All K. pneumoniae and E. coli transconjugants produced K. pneumoniae carbapenemase 2 (KPC-2), and most of them produced TEM, SHV and CTX-M. Additionally, 27 isolates and 27 E. coli transconjugants carried the qnr gene (25 were qnrB2 and 2 were qnrS1). K. pneumoniae harboured several plasmids, and bla(KPC-2) was located on a 55 kb plasmid. SDS-PAGE and ompK35/36 gene sequence analysis of OMPs suggested that porins in K. pneumoniae are expressed normally. The MICs of the carbapenems did not change in the presence of CCCP. Thus, production of KPC-2 appears to play an important role in resistance to carbapenems, although other mechanisms may be involved. The bla(KPC-2) gene is associated with several antibiotic-resistance genes, such as bla(TEM), bla(SHV), bla(CTX-M) and qnr.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Infecciones por Klebsiella / Carbapenémicos / Klebsiella pneumoniae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2011 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Bacterianas / Beta-Lactamasas / Infecciones por Klebsiella / Carbapenémicos / Klebsiella pneumoniae Tipo de estudio: Prevalence_studies / Risk_factors_studies Límite: Humans País/Región como asunto: Asia Idioma: En Revista: J Med Microbiol Año: 2011 Tipo del documento: Article Pais de publicación: Reino Unido