Competitive heterogeneous enzyme immunoassay for theophylline by flow-injection analysis with electrochemical detection of p-aminophenol.

Gil, E P; Tang, H T; Halsall, H B; Heineman, W R; Misiego, A S

Gil, E P; Tang, H T; Halsall, H B; Heineman, W R; Misiego, A S.

Afiliación

Gil EP; Department of Chemistry, University of Cincinnati, OH 45221-0172.

Clin Chem ; 36(4): 662-5, 1990 Apr.

Article en En | MEDLINE | ID: mdl-2138937

RESUMEN

A competitive enzyme-linked immunoabsorbent assay based on the flow-injection amperometric detection of p-aminophenol has been investigated with use of the materials and general procedure of a commercial kit for the determination of theophylline in human serum. The antibody is immobilized on glass beads, and the enzyme label is alkaline phosphatase (EC 3.1.3.1). The high currents generated during the electrochemical detection allowed a rapid (35 min) and simple determination of theophylline throughout its therapeutic range (10-20 mg/L) and also in the subtherapeutic range (detection limit of about 80 micrograms/L).

Asunto(s)

Aminofenoles/análisis; Ensayo de Inmunoadsorción Enzimática/instrumentación; Teofilina/sangre; Electroquímica; Electrodos; Humanos; Técnicas para Inmunoenzimas; Juego de Reactivos para Diagnóstico; Reología; Teofilina/uso terapéutico

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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Teofilina / Ensayo de Inmunoadsorción Enzimática / Aminofenoles Tipo de estudio: Diagnostic_studies Límite: Humans Idioma: En Revista: Clin Chem Asunto de la revista: QUIMICA CLINICA Año: 1990 Tipo del documento: Article Pais de publicación: Reino Unido

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