Accumulation of dodecyltriphenylphosphonium in mitochondria induces their swelling and ROS-dependent growth inhibition in yeast.
J Bioenerg Biomembr
; 43(2): 175-80, 2011 Apr.
Article
en En
| MEDLINE
| ID: mdl-21360288
Hydrophobic cations with delocalized charge are used to deliver drugs to mitochondria. However, micromolar concentrations of such compounds could be toxic due to their excessive accumulation in mitochondria. We studied possible pathophysiological effects of one such cation, i.e. dodecyltriphenylphosphonium (C(12)-TPP), in the yeast Saccharomyces cerevisiae. First, we found that C(12)-TPP induces high-amplitude mitochondrial swelling. The swelling can be prevented by addition of protonophorous uncoupler FCCP or antioxidant alpha-tocopherol, but not other tested antioxidants (N-acetylcysteine and Trolox). Second, FCCP prevents ROS-sensitive fluorescent dye (dichlorofluorescein diacetate) staining of yeast treated with C(12)-TPP. We also showed that all tested antioxidants partially restore the growth inhibited by C(12)-TPP. The latter points that ROS rather than the mitochondria swelling limit the growth rate.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Compuestos Organofosforados
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Saccharomyces cerevisiae
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Especies Reactivas de Oxígeno
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Mitocondrias
Idioma:
En
Revista:
J Bioenerg Biomembr
Año:
2011
Tipo del documento:
Article
País de afiliación:
Rusia
Pais de publicación:
Estados Unidos