Antioxidant activity, acetylcholinesterase inhibition, iridoid content and mutagenic evaluation of Leucosidea sericea.
Food Chem Toxicol
; 49(5): 1122-8, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21310209
Leucosidea sericea is an important medicinal plant widely used in traditional medicine in southern Africa. Leaf and stem petroleum ether (PE), dichloromethane (DCM) and 50% aqueous methanol (MeOH) extracts were investigated for antioxidant and acetylcholinesterase inhibitory activities. The safety of the extracts was evaluated using the Ames test. In addition, the iridoid content of L. sericea stems and leaves were quantified. For DPPH radical-scavenging activity, the stem MeOH extract (EC(50) value: 1.6 µg/ml) was more potent than ascorbic acid (EC(50) value: 1.7 µg/ml). In the ß-carotene-linoleic acid model system, antioxidant activity of the leaf DCM extract (89.8%) was not significantly different to that of butylated hydroxytoluene (BHT) (98.9%). All extracts showed a dose-dependent acetylcholinesterase inhibition; in terms of the IC(50) value, the leaf DCM extract (0.14 mg/ml) was the most potent sample. Total iridoid content was 35% higher in the stem extract than in the leaf extract. Based on the Ames test, L. sericea extracts were not mutagenic, either with or without S9 metabolic activation. These findings suggest the safety as well as the potential of L. sericea as a possible source of novel/alternative antioxidant and acetylcholinesterase inhibitory compounds.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
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Inhibidores de la Colinesterasa
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Rosaceae
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Iridoides
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Mutágenos
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Antioxidantes
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Food Chem Toxicol
Año:
2011
Tipo del documento:
Article
País de afiliación:
Sudáfrica
Pais de publicación:
Reino Unido