Modifying the antigen-immunization schedule improves the variety of monoclonal antibodies obtained from immune-phage antibody libraries against HIV-1 Nef and Vif.
J Biosci Bioeng
; 111(5): 597-9, 2011 May.
Article
en En
| MEDLINE
| ID: mdl-21277253
Immune phage antibody libraries are an attractive technology for isolating antigen-specific monoclonal antibodies (mAbs). Here we show that the immunization schedule affects the immune phage antibody library properties. We subcutaneously (s.c.) administered HIV-1 Nef and Vif antigens with different schedules (25 µg × 2 s.c. and 10 µg × 3 s.c.). The variety of isolated mAbs in 25 µg × 2 s.c. groups (Nef: 11 clones, Vif: 9 clones) was superior to that in the 10 µg × 3 s.c. groups (Nef: 2 clones, Vif: 1 clone). This finding suggests that it is important to optimize the immunization schedule for isolating a wide variety of mAbs.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Esquemas de Inmunización
/
Biblioteca de Péptidos
/
Productos del Gen nef del Virus de la Inmunodeficiencia Humana
/
Productos del Gen vif del Virus de la Inmunodeficiencia Humana
/
Anticuerpos Monoclonales
Límite:
Animals
Idioma:
En
Revista:
J Biosci Bioeng
Asunto de la revista:
ENGENHARIA BIOMEDICA
/
MICROBIOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
Japón
Pais de publicación:
Japón