Development and characterization of 60 novel EST-SSR markers in half-smooth tongue sole Cynoglossus semilaevis.

Sha, Z-X; Luo, X-H; Liao, X-L; Wang, S-L; Wang, Q-L; Chen, S-L

Sha, Z-X; Luo, X-H; Liao, X-L; Wang, S-L; Wang, Q-L; Chen, S-L.

Afiliación

Sha ZX; Key Lab for Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.

J Fish Biol ; 78(1): 322-31, 2011 Jan.

Article en En | MEDLINE | ID: mdl-21235563

RESUMEN

Sixty novel simple sequence repeat (SSR) markers were developed from expressed sequence tags (EST) of half-smooth tongue sole Cynoglossus semilaevis exploited in the laboratory. The number of alleles, observed and expected heterozygosity per locus ranged from two to 16, from 0·0833 to 1·0000 and from 0·0816 to 0·913, respectively. Of these SSRs, 20 had significant homology to known genes by BLASTx (basic local alignment search tool x) search. For cross-species amplification, there are 53 positive amplifications in Japanese flounder Paralichthys olivaceus with 12 polymorphic loci and 51 positive amplifications in Senegalese sole Solea senegalensis with 11 polymorphic loci. These new EST-SSR markers will be useful for genetic studies and genome mapping of C. semilaevis and its closely related fishes.

Asunto(s)

Etiquetas de Secuencia Expresada; Peces Planos/genética; Repeticiones de Minisatélite; Animales; Polimorfismo Genético

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Peces Planos / Repeticiones de Minisatélite / Etiquetas de Secuencia Expresada Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: J Fish Biol Año: 2011 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido

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