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Monolithic spin column extraction and GC-MS for the simultaneous assay of diquat, paraquat, and fenitrothion in human serum and urine.
Saito, Takeshi; Fukushima, Tomokazu; Yui, Yuko; Miyazaki, Shota; Nakamoto, Akihiro; Namera, Akira; Inokuchi, Sadaki.
Afiliación
  • Saito T; Department of Emergency and Critical Care Medicine, Tokai University School of Medicine, Shimokasuya 143, Isehara, Kanagawa 259-1143, Japan. saito@is.icc.u-tokai.ac.jp
Anal Bioanal Chem ; 400(1): 25-31, 2011 Apr.
Article en En | MEDLINE | ID: mdl-21221537
We present a method based on monolitic spin column extraction and gas chromatography-mass spectrometry as an analytical method for screening diquat (DQ), paraquat (PQ), and fenitrothion in serum and urine. This method is useful for clinical and forensic toxicological analyses. Recovery of DQ, PQ, and fenitrothion from serum and urine, spiked at concentrations between 0.1, 2.5, 20, and 45 µg/ml, ranged from 51.3% to 106.1%. Relative standard deviation percentages were between 3.3% and 14.8%. Detection and quantitation limits for serum and urine were 0.025 and 0.05 µg/ml, respectively, for DQ, 0.1 and 0.1 µg/ml, respectively, for PQ, and 0.025 and 0.05 µg/ml, respectively, for fenitrothion. Therefore, these compounds can be detected and quantified in the case of acute poisoning.
Asunto(s)

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraquat / Diquat / Fenitrotión / Cromatografía de Gases y Espectrometría de Masas Límite: Humans Idioma: En Revista: Anal Bioanal Chem Año: 2011 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Paraquat / Diquat / Fenitrotión / Cromatografía de Gases y Espectrometría de Masas Límite: Humans Idioma: En Revista: Anal Bioanal Chem Año: 2011 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Alemania