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[Cloning and expression of a fragment of Corylus heterophylla 1 and identifying its immunologic competence].
Li, Na; Hu, Dong-sheng; Liu, Zhi-gang; Lin, Si-li; Luo, Xin-ping; Huang, Zhong.
Afiliación
  • Li N; Department of Epidemiology and Statistics, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. lina475300@163.com
Article en Zh | MEDLINE | ID: mdl-21208553
AIM: To clone, express, and identify a fragment of Cor h 1 from Corylus heterophylla. METHODS: Through bioinformatics predication, the antigenic epitope of Cor h 1 was selected. A fragment gene of Cor h 1 was amplified by PCR and cloned into pMD18-T vector for sequencing. Then the fragment gene was sub cloned into pET-32a expression vector for expression, and then purified by metal (Ni(2+);) chelating affinity chromatography. The immunogenicity was tested by Western blot. RESULTS: The length of the fragment gene was 243 bp, coding 81 amino acids; the relative molecular mass of recombinant protein was 9 000. And the fragment of Cor h 1 was mainly expressed as soluble protein, purified protein has good immunogenicity. CONCLUSION: The fragment gene of Cor h 1 was successfully cloned and expressed in this study, and the recombinant protein possessed good IgE-binding capacity.
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Alérgenos / Corylus Idioma: Zh Revista: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2011 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Plantas / Alérgenos / Corylus Idioma: Zh Revista: Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2011 Tipo del documento: Article País de afiliación: China Pais de publicación: China