[Cloning and expression of a fragment of Corylus heterophylla 1 and identifying its immunologic competence].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 27(1): 4-6, 2011 Jan.
Article
en Zh
| MEDLINE
| ID: mdl-21208553
AIM: To clone, express, and identify a fragment of Cor h 1 from Corylus heterophylla. METHODS: Through bioinformatics predication, the antigenic epitope of Cor h 1 was selected. A fragment gene of Cor h 1 was amplified by PCR and cloned into pMD18-T vector for sequencing. Then the fragment gene was sub cloned into pET-32a expression vector for expression, and then purified by metal (Ni(2+);) chelating affinity chromatography. The immunogenicity was tested by Western blot. RESULTS: The length of the fragment gene was 243 bp, coding 81 amino acids; the relative molecular mass of recombinant protein was 9 000. And the fragment of Cor h 1 was mainly expressed as soluble protein, purified protein has good immunogenicity. CONCLUSION: The fragment gene of Cor h 1 was successfully cloned and expressed in this study, and the recombinant protein possessed good IgE-binding capacity.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Proteínas de Plantas
/
Alérgenos
/
Corylus
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Asunto de la revista:
ALERGIA E IMUNOLOGIA
Año:
2011
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
China